![]() Some isolates may carry 1–3 distinct satellite DNA molecules ( Yu et al., 2011). ![]() Except DNA-U3, each component encodes a protein with known function. The genome of BBTV contains six components, namely, DNA-R, DNA-U3, DNA-S, DNA-M, DNA-C, and DNA-N. Banana bunchy top virus (BBTV), belonging to the genus Babuvirus within family Nanoviridae, is known to infect all members of family Musaceae and is transmitted by aphids of the genus Pentalonia in a circulative manner ( Watanabe et al., 2013, 2016 Kumar et al., 2015). The family Nanoviridae comprises plant viruses that have very small virions with six to eight components, ranging in size from 1.0 to 1.1 kb each component is separately encapsulated inside an individual virion ( Yu et al., 2019b). Viruses that are members of the families Nanoviridae, Genomoviridae, and Geminiviridae have small genomes that replicate ssDNA using the rolling circle replication mechanism ( Schmid et al., 2014 Weitzman and Fradet-Turcotte, 2018 Li et al., 2020). In addition to the aforementioned viruses, the satellite ssDNA molecules of nanovirids, and geminiviruses, which are not necessary for infection, have been reclassified into Alphasatellitidae and Tolecusatellitidae families ( Zubair et al., 2017 Briddon et al., 2018). These viruses are Microviridae and Inoviridae infecting bacteria, Bacilladnaviridae infecting diatoms, Pleolipoviridae and Spiraviridae infecting archaea, Anelloviridae, Geminiviridae and Nanoviridae infecting plants, Circoviridae infecting birds and mammals, Genomoviridae infecting plants and animals, and Smacoviridae that infects both animals and humans ( Malathi and Dasgupta, 2019 Venkataraman and Selvarajan, 2019 Li et al., 2020). Currently, 11 families of ssDNA viruses have been established by the International Committee on Taxonomy of Viruses (ICTV, 2019 Release). In conclusion, this study proposes a new approach to obtain the genome of nanovirids, and provides insights for studying the transcriptional mechanism of the family Nanoviridae, Genomoviridae, and Geminiviridae.Ĭircular single-stranded DNA (ssDNA) viruses are widely distributed worldwide, infecting diverse hosts from all three domains of bacteria, archaea, and eukaryotes ( Lefkowitz et al., 2008). The RT-qPCR analysis indicated that the vmRNA levels of coding regions were 3.19–103.53 folds higher than those of non-coding regions, implying that the integrity of genome assembly depended on the transcription level of non-coding region. Assembly analysis of BBTV-derived reads indicated that the full-length sequences were obtained for DNA-R, DNA-U3, DNA-S, DNA-M, DNA-N, NewS2, and Sat4 components, while two gaps (73 and 25 nt) missing in the DNA-C component which was further filled by reverse transcription-PCR (RT-PCR). Accordingly, the near-complete genome of BBTV B2 isolate was assembled using transcriptome sequencing data from virus-infected banana leaves. In this study, transcriptome sequencing analysis showed that the full-length of BBTV each genomic component was transcribed into viral mRNA (vmRNA). ![]() ![]() However, RNA-based techniques to obtain the genome sequence of such multi-component viruses have not been reported. Its genome is usually amplified and obtained at the DNA level. Among these, the genome of Banana bunchy top virus (BBTV) comprises at least six circular, ssDNA components that are ∼1 kb in length. 2Hainan Key Laboratory of Tropical Microbe Resources, Haikou, ChinaĬircular single-stranded DNA (ssDNA) viruses are widely distributed globally, infecting diverse hosts ranging from bacteria, archaea, and eukaryotes.1Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.Nai-tong Yu 1,2*, Yu-liang Zhang 1, Jian-hua Wang 1,2 and Zhi-xin Liu 1,2* ![]()
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